Context: Periodontal disease is a disease, or more likely a number of diseases of the periodontal tissues that results in attachment loss and destruction of alveolar bone. The dental biofilm is responsible for the pathogenesis of the periodontal lesions, resulting both from the direct degradative action and the indirect inflammatory lesions, mediated by microbial cells and their virulence factors.
Aims:The aims of this prospective study were the isolation, identification and characterization of the antibiotic susceptibility profiles of bacterial isolated from patients with periodontal lesions.
Material and Methods: This prospective and non-randomized study was carried out at Department of Periodontology, MPCD & RC, Gwalior and Microbiology, G. R. M. C., Gwalior, Madhya Pradesh for the period of one and half years. The study was conducted for the period of 1 and half year. During the period a total 50 samples were collected following inclusion and exclusion criteria and processed as per Clinical and Laboratory Standards Institute guidelines. The statistical analysis was performed by SPSS software.
Results: A total of 50 study subjects were included, with culture positivity rate of 36% (18). Male to female ratio was 2:1 with mean age of 38.1 years. E. coli, Staphylococcus aureus, Streptococcus mutant, Klebsiella species and Candida species were isolated. Maximum resistant was observed against ceftriaxone (60%), ceftazidime (53%), amoxicillin clavulanate (46%) and low resistance against gentamicin, ciprofloxacin and doxycycline.
Conclusion: The antibiotic susceptibility assay revealed different profiles and significant levels of antimicrobial resistance, reflecting the necessity to perform the microbiological analysis and the antibiotic susceptibility testing in order to select the optimal antimicrobial therapy for the treatment of the periodontal disease.
